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* The calculation works plausibly when all params.PnB are the same In the main FlowJo workspace window, find the new concatenated file and open a plot of live, single cells 10. Memset(value, '\0', sizeof(char)*params.PnB/8) Int num_params = sizeof(params) / sizeof(params) Sorry to make it longer but additional information: typedef struct _fcs_parameter I just can't find any information online and I'm sure the makers of FlowJo would be unwilling to share the secret ). It may be something to do with how the data is transformed. for example the data value for sample A is stored in 16 bits.ī0 = (bytes & 0xff) PAR*2)+(j*params.PnB/8)), params.PnB/8) īut I still get a skewed data. Int PnB = 16 // bitwidth of data stored for a specific channel value I understand that data generated on MacOS 9 CellQuest is stored in big endian order and believe that I am correctly transforming the data as such: for (int i = 0 i
FLOWJO 10 OFFSET PLOT SOFTWARE
When I extract the data points for FSC-H and SSC-H I don't get the same results as I would on other flow software (namely FlowJo). The FCS sample data file I am using to test the software was generated with FACSCaliber on MacOS 9 CellQuest. I am working on an in-house flow analysis software and am running into some issues. This is incorrect, and not sure how I got this formula. Where PAR is the number of Parameters, PnB is the bitwidth, and i is from 0 to total events and j is from 0 to PAR. I initially had (i * PAR * 2) + (j * PnB/8) I have two for loops i & j, and I need to calculate the offset using that. For example, if I have 3 events and 4 parameters of bitwidth 8, the binary data 00 00 00 00 00 00 00 00 00 00 00 00 00 00 00 00 The binary data is "arranged" in a matrix. The odd result I get is that a couple of my parameters have all 0s when reading into flowCore despite these parameters having data when viewed in flowjo or other applications. The core of the problem is traversing the data, as Jonathan Leffler kind of alluded. Hi, I find that reading in fcs files exported as version 3.1 from Miltenyis Macsquantify is giving some unexpected results.